受付中Zasshikai seminar 1992nd, Prof. Hung-Wen Li, “Watching Protein-DNA Assembly in Action: Single-Molecule Views on Dynamics and Regulation Mechanisms”
Jul.23
- 日時
- 2025/7/23 16:00～
- 会場
- 5F Auditorium, Chemistry Main Bldg.
- 講師
- Prof. Hung-Wen Li（Department of Chemistry, National Taiwan University, Taiwan）
- 演目
- "Watching Protein-DNA Assembly in Action: Single-Molecule Views on Dynamics and Regulation Mechanisms"
- 担当
- Prof. Tatsuya Tsukuda (ext. 24363), Department of Chemistry, Graduate School of Science
Repairing DNA damage to maintain genome stability is essential for cell survival. Homologous recombination is the major process to repair damaged DNA double-strand breaks with high fidelity. The key to this process is the assembly of RAD51 and DMC1 recombinases onto damaged DNA strands. These proteins must assemble into specific structures, known as nucleoprotein filaments, to carry out their function. Additional accessory proteins regulate and stimulate this assembly process by stabilizing these filaments and directing the repair steps. Our lab uses single-molecule microscopy tools, including multi-color fluorescence colocalization, fluorescence resonance energy transfer (FRET), and optical tweezers, to directly observe how these protein-DNA complexes are assembled and regulated. By capturing these events one molecule at a time, we gain detailed insight into how DNA repair is coordinated. These findings help us understand the principles and regulatory strategies of molecular assembly in biological systems.
