受付中Zasshikai seminar 1989th, Professor Chong Fang, “Capturing Molecular Movies of Fluorescent Proteins and Biosensors with an Ultrafast Electronic and Raman Spectroscopic Platform”
May23
- 日時
- 2025/5/23 16:00～18:00
- 会場
- 5F Auditorium, Chemistry Main Bldg.
- 講師
- Professor Chong Fang (Oregon State University)
- 演目
- “Capturing Molecular Movies of Fluorescent Proteins and Biosensors with an Ultrafast Electronic and Raman Spectroscopic Platform”
- 担当
- Prof. Robert E Campbell (ext.26528) , Department of Chemistry, School of Science
Abstract
Structural dynamics of condensed-phase systems such as fluorescent proteins and biosensors
govern their functions which broadly impact the chemical, biological, and health fields. However, a
complete understanding of their working mechanisms is hindered by the lack of suitable tools that
can resolve atomic motions on intrinsic molecular timescales. This talk presents the table-top
wavelength-tunable femtosecond stimulated Raman spectroscopy (FSRS), aided by transient
absorption spectroscopy and quantum calculations/molecular dynamics simulations, which have
captured molecular movies of fluorescence and other nonradiative events like never before.
Representative examples include the green and yellow fluorescent protein (GFP/YFP) from
jellyfish and sea anemone that can be engineered to emit from blue, green to red and sense calcium
(Ca2+) and chloride ions (Cl–) in a ratiometric manner, revealing the ultrafast interplay between
excited-state events such as proton transfer, cis-trans isomerization, dark state formation, and
conformational trapping (rigidity) or switching (flexibility). Such mechanistic understandings have
propelled us to delineate the multidimensional excited- and ground-state potential energy surface
and rationally design/engineer biomolecular systems with strategic “microscopic” modifications
and improved or novel “macroscopic” functions.
