受付中Zasshikai seminar 1997th, Associate professor Fabrice MICHEL, “Flow Chemistry Concepts and Techniques Applied to Solid Phase Oligonucleotide Synthesis”
Jul.8
- 日時
- 2025/7/8 16:00〜
- 会場
- Class room 284, School of Science Bldg. 1 (East)
- 講師
- Associate professor Fabrice MICHEL (University of Strasbourg, Faculty of Life Sciences, France)
- 演目
- “Functional assembly of RNA Polymerase IV machinery : Role of associated proteins for small RNA biogenesis and transposon silencing in plants”
- 担当
- Prof. Takeaki Ozawa (ext.24351), Department of Chemistry, Graduate School of Science
Abstract:Maintaining genome integrity is necessary for the survival of organisms.
Genomes face continuous threats not only from external mutagenic factors
but also intrinsically from mobile genetic elements known as
transposable elements (TEs). Although potentially deleterious, TEs are a
major component of plant and animal genomes and have contributed to
genome evolution through their mobility.
The RdDM (RNA-directed DNA methylation) pathway inhibits TE expression
in plants. This inactivation also extends to adjacent genes, leading to
transcriptional repression that is propagated over several generations
via so-called epigenetic mechanisms. RNA polymerase IV (Pol IV)
initiates the RdDM pathway by transcribing TEs in the nucleus. The
resulting non-coding RNAs are recognised by the RNA-dependent RNA
polymerase RDR2, which generates double-stranded RNAs (dsRNAs). These
are then cut into small interfering RNAs (siRNAs) of 24 nucleotides that
guide DNA methylation towards TEs throughout the genome.
This talk will focus on the functional assembly of the RNA Polymerase IV
(Pol IV) complex in plants, emphasizing the roles of associated proteins
in small RNA biogenesis and transposon silencing. Recent insights into
the molecular interactions that guide Pol IV recruitment and activity
will also be presented.
