Preprint. Synthesis and application of a photocaged L-lactate

Preprint. A lactate-dependent shift of glycolysis mediates synaptic and cognitive processes

Preprint. A red fluorescent genetically encoded biosensor for extracellular L-lactate

Preprint. A blue-shifted genetically encoded Ca²⁺ indicator with enhanced two-photon absorption

Preprint. Far-red fluorescent genetically encoded calcium ion indicators

Preprint. An optogenetic method for the controlled release of single molecules

139. High performance genetically-encoded green fluorescent biosensors for intracellular L-lactate

138. タンパク質を基盤とする蛍光イメージングセンサー開発の最前線 [Development of fluorescent imaging sensors based on proteins]

137. A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein

136. Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging

135. Construction of the lactate-sensing fibermats by confining sensor fluorescent protein of lactate inside nanofibers of the poly(HPMA/DAMA)/ADH-nylon 6 core-shell fibremat

134. Rational engineering of an improved genetically encoded pH sensor based on superecliptic pHluorin

Y. Shen*, Y. Wen, S. Sposini, A.A. Vishwanath, A.S. Abdelfattah, E.R. Schreiter, M.J. Lemieux, J. de Juan-Sanz, D. Perrais, and R.E. Campbell*, ”Rational engineering of an improved genetically encoded pH sensor based on superecliptic pHluorin”, ACS Sens., 2023, 8, 3014–3022.

133. Maximizing the performance of fluorescent protein-based biosensors

132. Directed Evolution of a Genetically Encoded Bioluminescent Ca2+ Sensor

131. Biosensor optimization using a FRET pair based on mScarlet red fluorescent protein and an mScarlet-derived green fluorescent protein

130. Chemigenetic indicators based on synthetic chelators and green fluorescent protein

128. The Endoplasmic Reticulum (ER) Kinase PERK Requires the Oxidoreductase ERO1 to Metabolically Adapt Mitochondria

126. A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life

124. Cyan fluorescent proteins derived from mNeonGreen

122. Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

120. Barcodes, co-cultures, and deep learning take genetically encoded biosensor multiplexing to the nth degree

119. A genetically encoded fluorescent biosensor for extracellular L-lactate

118. Design and prototyping of genetically encoded arsenic biosensors based on transcriptional regulator AfArsR

116. Structure- and mechanism-guided design of single fluorescent protein-based biosensors

115. Controlled Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Dexamethasone-Loaded Light-Responsive Microgels

114. Switching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based Ca2+ Biosensor

112. Engineering photosensory modules of non-opsin-based optogenetic actuators

110. Challenges for therapeutic applications of opsin-based optogenetic tools in humans

108. A bright and high-performance genetically encoded Ca2+ indicator based on mNeonGreen fluorescent protein

106. Engineering genetically encoded fluorescent indicators for imaging of neural activity: progress and prospects

104. Ratiometric Detection of Nerve Agents by Coupling Complementary Properties of Silicon-Based Quantum Dots and Green Fluorescent Protein

102. Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators

100. A genetically encoded near-infrared fluorescent calcium ion indicator

Y. Qian (equal contribution), K.D. Piatkevich (equal contribution), B. McLarney (equal contribution), A.S. Abdelfattah, S. Mehta, M.H. Murdock, S. Gottschalk, R.S. Molina, W. Zhang, Y. Chen, J. Wu, M. Drobizhev, T.E. Hughes, J. Zhang, E.R. Schreiter, S. Shoham, D. Razansky, E.S. Boyden, and R.E. Campbell*, “A genetically encoded near-infrared fluorescent calcium ion indicator”, Nat. Methods, 2019, 16, 171–174.

98. A bioluminescent Ca²⁺ indicator based on a topological variant of GCaMP6s

Y. Qian, V. Rancic, J. Wu, K. Ballanyi, and R.E. Campbell* , “A bioluminescent Ca²⁺ indicator based on a topological variant of GCaMP6s”, ChemBioChem, 2019, 20, 516-520.

96. In vivo photoacoustic difference-spectra imaging of bacteria using photoswitchable chromoproteins

R.K.W. Chee, Y. Li, W. Zhang, R.E. Campbell, and R.J. Zemp*, “In vivo photoacoustic difference-spectra imaging of bacteria using photoswitchable chromoproteins”, J. Biomed. Opt., 2018, 23, 106006.

94. Unnaturally aglow with a bright inner light

Y. Nasu and Robert E. Campbell*, “Unnaturally aglow with a bright inner light“, Science, 2018, 359, 868-869. [not peer reviewed]

92. Enhancing fluorescent protein photostability through robot assisted photobleaching

M.D. Wiens, F. Hoffmann, Y. Chen, and R.E. Campbell*, “Enhancing fluorescent protein photostability through robot assisted photobleaching”, Integr. Biol., 2018, 10, 419-428.

90. Genetically Encoded Glutamate Indicators with Altered Color and Topology

J. Wu, A.S. Abdelfattah, H. Zhou, A. Ruangkittisakul, Y. Qian, K. Ballanyi and R.E. Campbell*, “Genetically Encoded Glutamate Indicators with Altered Color and Topology”, ACS Chem. Biol., 2018, 13, 1832–1837.

88. Surveying the landscape of optogenetic methods for detection of protein-protein interactions

M.D. Wiens and R.E. Campbell*, “Surveying the landscape of optogenetic methods for detection of protein-protein interactions”, Wiley Interdiscip. Rev. Syst. Biol. Med., 2018, 10, e1415.

86. Illuminating Photochemistry of an Excitation Ratiometric Fluorescent Protein Calcium Biosensor

L. Tang, Y. Wang, W. Liu, Y. Zhao, R.E. Campbell, and C. Fang*, “Illuminating Photochemistry of an Excitation Ratiometric Fluorescent Protein Calcium Biosensor”, J. Phys. Chem. B, 2017, 121, 3016–3023.

84. Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

Y. Shen, Y. Chen, J. Wu, N.C. Shaner, and R.E. Campbell*, “Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity”, PLoS ONE, 2017, 12, e0171257.

82. Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes

A.S. Abdelfattah, V. Rancic, B. Rawal, K. Ballanyi, and R.E. Campbell*, “Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes”, ChemComm, 2016, 52, 14153–14156.

80. The growing and glowing toolbox of fluorescent and photoactive proteins

E.A. Rodriguez*, R.E. Campbell*, J.Y. Lin*, M.Z. Lin*, A. Miyawaki*, A.E. Palmer*, X. Shu*, J. Zhang* and R.Y. Tsien*, “The growing and glowing toolbox of fluorescent and photoactive proteins”, Trends Biochem. Sci. 2017, 42, 111–129.

78. Spying on Cells: Toward a Perfect Sleeper Agent

M.D. Wiens, X. Lu, and R.E. Campbell*, “Spying on Cells: Toward a Perfect Sleeper Agent”. Cell Chem. Biol. 2016, 23, 756-758.

76. Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging

Y. Li (equal contribution), A. Forbrich (equal contribution), J. Wu, P. Shao, R. E. Campbell* and R. Zemp*, “Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging”. Sci. Rep., 2016, 6,  22129.

74. Altered E. coli membrane protein assembly machinery allows proper membrane assembly of eukaryotic protein vitamin K epoxide reductase

F. Hatahet, J.L. Blazyk, E. Martineau, E. Mandela, Y. Zhao, R.E. Campbell, J. Beckwith* and D. Boyd, “Altered E. coli membrane protein assembly machinery allows proper membrane assembly of eukaryotic protein vitamin K epoxide reductase”. Proc. Natl. Acad. Sci. U.S.A. 2015, 112, 15184–15189.

72. Fluorescent proteins for neuronal imaging

Y. Zhao and R.E. Campbell*, “Fluorescent proteins for neuronal imaging”, in New techniques in systems neuroscience, Ed. A. Douglass. Springer International Publishing, Switzerland, April 2015, pages 57-96. [ISBN: 978-3-319-12912-9]

70. Emerging fluorescent protein technologies

J.R. Enterina, L. Wu, and R.E. Campbell*, “Emerging fluorescent protein technologies”, Curr. Opin. Chem. Biol., 2015, 27, 10–17.

68. Fluorescent biosensors illuminate calcium levels within defined beta-cell endosome subpopulations

T. Albrecht (equal contribution), Y. Zhao (equal contribution), T.H. Nguyen, R.E. Campbell, and J.D. Johnson*, “Fluorescent biosensors illuminate calcium levels within defined beta-cell endosome subpopulations”, Cell Calcium, 2015, 57, 263-274. [Open access]

66. A Photochromic and Thermochromic Fluorescent Protein

Y. Shen, M.D. Wiens, and R.E. Campbell*, “A Photochromic and Thermochromic Fluorescent Protein”. RSC Adv., 2014, 4, 56762-56765. [Open Access PDF]

64. A long Stokes shift red fluorescent protein Ca2+ indicator for 2-photon and ratiometric imaging

J. Wu, A.S. Abdelfattah, L.S. Miraucourt, E. Kutsarova, A. Ruangkittisakul, H. Zhou, K. Ballanyi, G. Wicks, M. Drobizhev, A. Rebane, E.S. Ruthazer, and R.E. Campbell*, “A long Stokes shift red fluorescent protein Ca2+ indicator for 2-photon and ratiometric imaging”, Nat. Commun., 2014, 5, 5262. [Supplementary Material; Funding from NSERC Discovery, CIHR MOP 123514, and a Vanier Canada Graduate and Alberta Innovates Health Solutions (AIHS) Scholarships to A.S.A]

62. Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum

J. Wu, D.L. Prole, Y. Shen, Z. Lin, A. Gnanasekaran, Y. Liu, L. Chen, H. Zhou, S.R.W. Chen, Y.M. Usachev, C.W. Taylor, and R.E. Campbell*, “Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum”, Biochem. J., 2014, 464, 13–22. [Supplementary Material; Funding from NSERC Discovery, CIHR MOP 123514, and a graduate scholarship from Alberta Innovates to Y.S.]

60. Excited state structural dynamics of a dual-emission calmodulin-green fluorescent protein sensor for calcium ion imaging

B.G. Oscar, W. Liu, Y. Zhao, L. Tang, Y Wang, R.E. Campbell, and C. Fang*, “Excited state structural dynamics of a dual-emission calmodulin-green fluorescent protein sensor for calcium ion imaging”, Proc. Natl. Acad. Sci. U.S.A., 2014, 111, 10191–10196. [Supplementary Material; Funding from NSERC Discovery, CIHR MOP 123514, and graduate scholarships from the University of Alberta and Alberta Innovates to Y.Z.]

58. Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response

Y. Zhao, A.S. Abdelfattah, Y. Zhao, A. Ruangkittisakul, K. Ballanyi, R.E. Campbell*, D.J. Harrison*, “Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response”, Integr. Biol. (Camb), 2014, 6(7), 714-725. [Open Access PDF; Supplementary material, Movie 1, Movie 2; Funding from NSERC Discovery, CIHR MOP 123514, and graduate scholarships from the University of Alberta (Y.Z) and Alberta Innovates to (Y.Z. and A.S.A)]

56. Optimization of a Genetically Encoded Biosensor for Cyclin B1-Cyclin Dependent Kinase 1 

A.S.F. Belal, B.R. Sell, H. Hoi, M.W. Davidson, and R.E. Campbell*, “Optimization of a Genetically Encoded Biosensor for Cyclin B1-Cyclin Dependent Kinase 1”. Mol. Biosyst., 2014, 10(2), 191-195. [Open Access PDF; Supplementary material; Funded by NSERC]

54. An engineered monomeric Zoanthus sp. yellow fluorescent protein

H. Hoi, E.S. Howe, Y. Ding, W. Zhang, M.A. Baird, B.R. Sell, J.R. Allen, M.W. Davidson, and R.E. Campbell*, “An engineered monomeric Zoanthus sp. yellow fluorescent protein”, Chem. Biol., 2013, 20, 1296-1304. [Highlighted in the same issue; Supplementary Material; Funded by NSERC Discovery and Alberta Innovates Technology Futures (AITF) Scholarship to W.Z.]

52. Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry

H.J. Carlson and R.E. Campbell*, “Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry”, Protein Eng. Des. Sel., 2013, 26(12): 763-772. [Supplementary Material; Funded by NSERC Discovery, NSERC PGSM, and Alberta Ingenuity Scholarship]

50. Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications

J. Wu, L. Liu, T. Matsuda, Y. Zhao, A. Rebane, M. Drobizhev, Y-F. Chang, S. Araki, Y. Arai, K. March, T. E. Hughes, K. Sagou, T. Miyata, T. Nagai*, W-h. Li*, R. E. Campbell*, “Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications”, ACS Chem. Neurosci., 2013, 4(6), 963-972. [Supplementary Material; Funded by CIHR NHG 99085, CIHR MOP 123514, NSERC Discovery, and Alberta Ingenuity Nanotechnology Scholarship to Y.Z.; Highlighted at OpenOptogenetics]

48. Optogenetic Reporters

S.C. Alford, J. Wu, Y. Zhao, R.E. Campbell, and T. Knöpfel*, “Optogenetic Reporters”. Biol. Cell., 2013, 105, 14-29. [Funded by CIHR NHG 99085, NSERC Discovery, NSERC CGSD3 to S.C.A., Alberta Ingenuity Ph.D. Scholarship to S.C.A., and Alberta Ingenuity Nanotechnology Scholarship to Y.Z.; Highlighted at ChemistryViews]

46. Dimerization-Dependent Green and Yellow Fluorescent Proteins

S.C. Alford, Y. Ding, T. Simmen, and R.E. Campbell*, “Dimerization-Dependent Green and Yellow Fluorescent Proteins”. ACS Synth. Biol., 2012, 1(12), 569-575. [Cover art; Supplementary Material;Author Feature; Funded by CIHR NHG 99085, NSERC Discovery, NSERC CGSD3 to S.C.A., and Alberta Ingenuity Ph.D. Scholarship to S.C.A]

44. Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells

P. Tewson, M. Westenberg, Y. Zhao, R.E. Campbell, A.M. Quinn, T.E. Hughes,* “Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells”. PLoS ONE, 2012, 7(8): e42791. [Open Access PDF; Funded by CIHR NHG 99085 and Alberta Ingenuity Nanotechnology Scholarship to Y.Z.]

42. Supramolecular hosts that recognize methyllysines and disrupt the interaction between a modified histone tail and its epigenetic reader protein

K.D. Daze,  T. Pinter,  C.S. Beshara,  A. Ibraheem,  S.A. Minaker,  M.C.F. Ma,  R.J.M. Courtemanche,  R.E. Campbell, and F. Hof*, “Supramolecular hosts that recognize methyllysines and disrupt the interaction between a modified histone tail and its epigenetic reader protein”. Chem. Sci., 2012, 3, 2695-2699. [Supplementary Material; Funded by Alberta Cancer Board and NSERC Discovery]

40. FRET-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells

Y. Ding, H-w. Ai, H. Hoi, R.E. Campbell*, “FRET-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells”. Anal. Chem., 2011, 83, 9687–9693. [Supplementary Material; Funded by CIHR NHG 94487 and 99085 and NSERC Discovery]

38. An Expanded Palette of Genetically Encoded Ca2+ Indicators

Y. Zhao, S. Araki, J. Wu, T. Teramoto, Y-F. Chang, M. Nakano, A.S. Abdelfattah, M. Fujiwara, T. Ishihara, T. Nagai, and R.E. Campbell*, “An Expanded Palette of Genetically Encoded Ca2+ Indicators”, Science, 2011, 333, 1888-1891. [Press release; Highlighted by C&EN Concentrates, Biophotonics, and Science Signaling; Supplementary Material; Funded by CIHR NHG 94487 and 99085, NSERC Discovery, Alberta Ingenuity Nanotechnology Scholarship to Y.Z., and Vanier CGS to A.S.A.]

36. Circularly permuted monomeric red fluorescent proteins with new termini in the β-sheet

H.J. Carlson, D. Cotton, and R.E. Campbell*, “Circularly permuted monomeric red fluorescent proteins with new termini in the β-sheet”, Protein Sci., 2010, 19, 1490-1499. [Funded by NSERC Discovery, NSERC USRA to D.C., NSERC PGSM to H.J.C., and Alberta Ingenuity Scholarship to H.J.C.]

34. Designs and applications of fluorescent protein-based biosensors

A. Ibraheem and R.E. Campbell*, “Designs and applications of fluorescent protein-based biosensors”, Curr. Opin. Chem. Biol., 2010, 14, 30-36. [Funded by Alberta Cancer Board]

32. Engineered fluorescent proteins: innovations and applications

M.W. Davidson and R.E. Campbell*, “Engineered fluorescent proteins: innovations and applications”, Nat. Methods, 2009, 6, 713-717. [Invited Commentary for 5th Anniversary issue.]

30. Red fluorescent protein pH biosensor to detect concentrative nucleoside transport

D.E. Johnson, H-w. Ai, P. Wong, J.D. Young, R.E. Campbell*, and J.R. Casey* “Red fluorescent protein pH biosensor to detect concentrative nucleoside transport”, J. Biol. Chem., 2009, 284, 20499-20511. [Supplementary Material]

28. An engineered tryptophan zipper-type peptide as a molecular recognition scaffold

Z. Cheng and R.E. Campbell*, “An engineered tryptophan zipper-type peptide as a molecular recognition scaffold”, J. Pept. Sci., 2009, 15, 523-532. [Supplementary Material]

26. Fluorescent proteins

R.E. Campbell*, “Fluorescent proteins”, Scholarpedia J., 2008, 3(7), 5410. [Open access; Article accessed more than 95,000 times as of July 2014]

24. Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

H-w. Ai, S.G. Olenych, P. Wong, M.W. Davidson, and R.E. Campbell*, “Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging”, BMC Biol., 2008, 6, 13. [Open Access PDF; Designated as a Highly Accessed article]

22. Computational prediction of absorbance maxima for a structurally diverse series of engineered green fluorescent protein chromophores

Q.K. Timerghazin, H.J. Carlson, C. Liang, R.E. Campbell,* and A. Brown*, “Computational prediction of absorbance maxima for a structurally diverse series of engineered green fluorescent protein chromophores”, J. Phys. Chem B, 2008, 112, 2533-2541. [Supplementary Material]

20. More than just pretty colors: the growing impact of fluorescent proteins in the life sciences

H-w. Ai and R.E. Campbell*, “More than just pretty colors: the growing impact of fluorescent proteins in the life sciences”, Biotechnology Focus (Bioscienceworld), 2007, issue 11, 16-18.

18. Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins

H.-w. Ai, N.C. Shaner, Z. Cheng, R.Y. Tsien, and R.E. Campbell*, “Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins”, Biochemistry, 2007,46, 5904 – 5910. [Featured on the cover of the June 2007 issue of Biophotonics]

16. Fluorescence-based characterization of genetically encoded peptides that fold in live cells: progress towards a generic hairpin scaffold

Z. Cheng and R.E. Campbell*, “Fluorescence-based characterization of genetically encoded peptides that fold in live cells: progress towards a generic hairpin scaffold”, Proc. SPIE, 2007, 6449, 64490S.

14. Assessing the Structural Stability of Designed β-Hairpin Peptides in the Cytoplasm of Live Cells 

Z. Cheng and R.E. Campbell*, “Assessing the Structural Stability of Designed β-Hairpin Peptides in the Cytoplasm of Live Cells”, ChemBioChem, 2006, 7, 1147-1150.

Postdoctoral research at the University of California, San Diego:

11. Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma red fluorescent protein

N.C. Shaner, R.E. Campbell, P.A. Steinbach, B.N.G. Giepmans, A.E. Palmer, and R.Y. Tsien*, “Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma red fluorescent protein”, Nat. Biotechnol., 2004, 22, 1567-1572.

9. A Monomeric Red Fluorescent Protein

R.E. Campbell, O. Tour, A.E. Palmer, P.A. Steinbach, G.S. Baird, D.A. Zacharias and R.Y. Tsien*, “A Monomeric Red Fluorescent Protein”, Proc. Natl. Acad. Sci. U.S.A., 2002, 99, 7877-7882.

7. Reducing the Environmental Sensitivity of Yellow Fluorescent Protein: Mechanism and Applications

O. Griesbeck, G.S. Baird, R.E. Campbell, D.A. Zacharias and R.Y. Tsien*, “Reducing the Environmental Sensitivity of Yellow Fluorescent Protein: Mechanism and Applications”, J. Biol. Chem., 2001, 276, 29188-29194.

6. The Structure of UDP-N-Acetylglucosamine 2-Epimerase Reveals Homology to Phosphoglycosyl Transferases

R.E. Campbell, S.C. Mosimann, M.E. Tanner*, and N.C.J. Strynadka*, “The Structure of UDP-N-Acetylglucosamine 2-Epimerase Reveals Homology to Phosphoglycosyl Transferases”,Biochemistry, 2000, 39, 14993-15001.

4. UDP-Glucose Analogues as Inhibitors and Mechanistic Probes of UDP-Glucose Dehydrogenase

R.E. Campbell and M.E. Tanner*, “UDP-Glucose Analogues as Inhibitors and Mechanistic Probes of UDP-Glucose Dehydrogenase”, J. Org. Chem., 1999, 64, 9487-9492.

2. Uridine diphospho-alpha-D-gluco-hexodialdose: Synthesis and kinetic competence in the reaction catalyzed by UDP-glucose dehydrogenase

R.E. Campbell and M.E. Tanner*, “Uridine diphospho-alpha-D-gluco-hexodialdose: Synthesis and kinetic competence in the reaction catalyzed by UDP-glucose dehydrogenase”, Angew. Chem. Int. Ed. Eng. 1997, 36, 1520-1522.