Refereed Papers

    2020
  • 1.
    S. Biselli, I. Alencastre, K. Tropmann, D. Erdmann, M. Chen, T. Littmann, A. F. Maia, M. Gomez-Lazaro, M. Tanaka, T. Ozawa, M. Keller, M. Lamghari, A. Buschauer, G. Bernhardt, Fluorescent H undefinedtextrm2 Receptor Squaramide-Type Antagonists: Synthesis, Characterization, and Applications. ACS Medicinal Chemistry Letters. 11, 1521–1528 (2020).
    DOI:10.1021/acsmedchemlett.0c00033
  • 1.
    T. Nishiguchi, H. Yoshimura, R. S. Kasai, T. K. Fujiwara, T. Ozawa, ACS Chemical Biology, in press, doi:10.1021/acschembio.0c00631.
    DOI:10.1021/acschembio.0c00631
  • 2019
  • 1.
    R. Shimada, T. Nakamura, T. Ozawa, Parallelized shifted‐excitation Raman difference spectroscopy for fluorescence rejection in a temporary varying system. Journal of Biophotonics. 12, e201960028 (2019).
    DOI:10.1002/jbio.201960028
  • 1.
    A. Takamura, D. Watanabe, R. Shimada, T. Ozawa, Comprehensive modeling of bloodstain aging by multivariate Raman spectral resolution with kinetics. Communications Chemistry. 2, 115 (2019).
    DOI:10.1038/s42004-019-0217-1
  • 1.
    N. Noda, T. Ishimoto, H. Mori, T. Ozawa, Enhanced bioluminescent sensor for longitudinal detection of CREB activation in living cells. Photochemical &Photobiological Sciences. 18, 2740–2747 (2019).
    DOI:10.1039/C9PP00249APubMed:31573014
  • 1.
    M. Endo, T. Iwawaki, H. Yoshimura, T. Ozawa, Photocleavable Cadherin Inhibits Cell-to-Cell Mechanotransduction by Light. ACS Chemical Biology. 14, 2206–2214 (2019).
    DOI:10.1021/acschembio.9b00460PubMed:31503442
  • 1.
    E. Bartole, T. Littmann, M. Tanaka, T. Ozawa, A. Buschauer, G. Bernhardt, [ 3 H]UR-DEBa176: A 2,4-Diaminopyrimidine-Type Radioligand Enabling Binding Studies at the Human, Mouse, and Rat Histamine H 4 Receptors. Journal of Medicinal Chemistry. 62, 8338–8356 (2019).
    DOI:10.1021/acs.jmedchem.9b01342
  • 1.
    M. ENDO, M. MIYASAKI, Q. LI, G. KAWAMURA, T. OZAWA, A Detection Method for GLUT4 Exocytosis Based on Spontaneous Split Luciferase Complementation. Analytical Sciences. 35, 835–838 (2019).
    DOI:10.2116/analsci.19C003
  • 1.
    A. Takamura, L. Halamkova, T. Ozawa, I. K. Lednev, Phenotype Profiling for Forensic Purposes: Determining Donor Sex Based on Fourier Transform Infrared Spectroscopy of Urine Traces. Analytical Chemistry. 91, 6288–6295 (2019).
    DOI:10.1021/acs.analchem.9b01058
  • 1.
    T. Morimoto, L. Chiu, H. Kanda, H. Kawagoe, T. Ozawa, M. Nakamura, K. Nishida, K. Fujita, T. Fujikado, Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction. The Analyst. 144, 2531–2540 (2019).
    DOI:10.1039/C8AN02213EPubMed:30839952
  • 1.
    J. Matsuo, S. Haga, K. Hashimoto, T. Okubo, T. Ozawa, M. Ozaki, H. Yamaguchi, Activation of caspase-3 during Chlamydia trachomatis -induced apoptosis at a late stage. Canadian Journal of Microbiology. 65, 135–143 (2019).
    DOI:10.1139/cjm-2018-0408
  • 2018
  • 1.
    T. Littmann, T. Ozawa, C. Hoffmann, A. Buschauer, G. Bernhardt, A split luciferase-based probe for quantitative proximal determination of Gαq signalling in live cells. Scientific Reports. 8, 17179 (2018).
    DOI:10.1038/s41598-018-35615-wPubMed:30464299
  • 1.
    H. Yonezawa, S. Tashiro, T. Shiraogawa, M. Ehara, R. Shimada, T. Ozawa, M. Shionoya, Preferential Photoreaction in a Porous Crystal, Metal–Macrocycle Framework: Pd II -Mediated Olefin Migration over [2+2] Cycloaddition. Journal of the American Chemical Society. 140, 16610–16614 (2018).
    DOI:10.1021/jacs.8b08534
  • 1.
    G. Kawamura, M. Hattori, K. Takamatsu, T. Tsukada, Y. Ninomiya, I. Benjamin, P. Sassone-Corsi, T. Ozawa, T. Tamaru, Cooperative interaction among BMAL1, HSF1, and p53 protects mammalian cells from UV stress. Communications Biology. 1, 204 (2018).
    DOI:10.1038/s42003-018-0209-1PubMed:30480104
  • 1.
    A. Takamura, K. Watanabe, T. Akutsu, T. Ozawa, Soft and Robust Identification of Body Fluid Using Fourier Transform Infrared Spectroscopy and Chemometric Strategies for Forensic Analysis. Scientific Reports. 8, 8459 (2018).
    DOI:10.1038/s41598-018-26873-9
  • 1.
    O. Takenouchi, H. Yoshimura, T. Ozawa, Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers. Scientific Reports. 8, 677 (2018).
    DOI:10.1038/s41598-017-19130-y
  • 1.
    N. Noda, T. Ozawa, Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B. Photochemistry and Photobiology. 94, 1071–1076 (2018).
    DOI:10.1111/php.12955
  • 1.
    Q. Li, H. Yoshimura, M. Komiya, K. Tajiri, M. Uesugi, Y. Hata, T. Ozawa, A robust split-luciferase-based cell fusion screening for discovering myogenesis-promoting molecules. The Analyst. 143, 3472–3480 (2018).
    DOI:10.1039/C8AN00285APubMed:29944152
  • 1.
    A. Schramm, P. Mueller-Thuemen, T. Littmann, M. Harloff, T. Ozawa, J. Schlossmann, Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies. International Journal of Molecular Sciences. 19, 1180 (2018).
    DOI:10.3390/ijms19041180
  • 1.
    S. Haga, A. Kanno, T. Ozawa, N. Morita, M. Asano, M. Ozaki, Detection of Necroptosis in Ligand-Mediated and Hypoxia-Induced Injury of Hepatocytes Using a Novel Optic Probe-Detecting Receptor-Interacting Protein (RIP)1/RIP3 Binding. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics. 26, 503–513 (2018).
    DOI:10.3727/096504017X15005102445191
  • 1.
    S. Haga, T. Ozawa, N. Morita, M. Asano, S. Jin, Yimin, M. Ozaki, Photo-Activatable Akt Probe: A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics. 26, 467–472 (2018).
    DOI:10.3727/096504017X15040166233313
  • 2017
  • 1.
    N. Noda, R. Awais, R. Sutton, M. Awais, T. Ozawa, Dynamic monitoring of p53 translocation to mitochondria for the analysis of specific inhibitors using luciferase-fragment complementation. Biotechnology and Bioengineering. 114, 2818–2827 (2017).
    DOI:10.1002/bit.26407
  • 1.
    H. J. Bailes, N. Milosavljevic, L.-Y. Zhuang, E. J. Gerrard, T. Nishiguchi, T. Ozawa, R. J. Lucas, Optogenetic interrogation reveals separable G-protein-dependent and -independent signalling linking G-protein-coupled receptors to the circadian oscillator. BMC Biology. 15, 40 (2017).
    DOI:10.1186/s12915-017-0380-8
  • 1.
    L. Chiu, S.-H. Ho, R. Shimada, N.-Q. Ren, T. Ozawa, Rapid in vivo lipid/carbohydrate quantification of single microalgal cell by Raman spectral imaging to reveal salinity-induced starch-to-lipid shift. Biotechnology for Biofuels. 10, 9 (2017).
    DOI:10.1186/s13068-016-0691-y
  • 1.
    M. Komiya, A. Ito, M. Endo, D. Hiruma, M. Hattori, H. Saitoh, M. Yoshida, T. Ozawa, A genetic screen to discover SUMOylated proteins in living mammalian cells. Scientific Reports. 7, 17443 (2017).
    DOI:10.1038/s41598-017-17450-7
  • 1.
    A. Takamura, K. Watanabe, T. Akutsu, H. Ikegaya, T. Ozawa, Spectral Mining for Discriminating Blood Origins in the Presence of Substrate Interference via Attenuated Total Reflection Fourier Transform Infrared Spectroscopy: Postmortem or Antemortem Blood? Analytical Chemistry. 89, 9797–9804 (2017).
    DOI:10.1021/acs.analchem.7b01756
  • 1.
    K. K. Kuhn, T. Littmann, S. Dukorn, M. Tanaka, M. Keller, T. Ozawa, G. Bernhardt, A. Buschauer, In Search of NPY Y 4 R Antagonists: Incorporation of Carbamoylated Arginine, Aza-Amino Acids, or d -Amino Acids into Oligopeptides Derived from the C-Termini of the Endogenous Agonists. ACS Omega. 2, 3616–3631 (2017).
    DOI:10.1021/acsomega.7b00451
  • 1.
    A. Rapakousiou, R. Sakamoto, R. Shiotsuki, R. Matsuoka, U. Nakajima, T. Pal, R. Shimada, A. Hossain, H. Masunaga, S. Horike, Y. Kitagawa, S. Sasaki, K. Kato, T. Ozawa, D. Astruc, H. Nishihara, Liquid/Liquid Interfacial Synthesis of a Click Nanosheet. Chemistry - A European Journal. 23, 8443–8449 (2017).
    DOI:10.1002/chem.201700201
  • 1.
    T. Tahara, T. Kanno, Y. Arai, T. Ozawa, Single-shot phase-shifting incoherent digital holography. Journal of Optics. 19, 065705 (2017).
    DOI:10.1088/2040-8986/aa6e82
  • 1.
    Y. Fukutani, J. Ishii, A. Kondo, T. Ozawa, H. Matsunami, M. Yohda, Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae. Biotechnology and Bioengineering. 114, 1354–1361 (2017).
    DOI:10.1002/bit.26255
  • 1.
    L. Chiu, T. Ichimura, T. Sekiya, H. Machiyama, T. Watanabe, H. Fujita, T. Ozawa, K. Fujita, Protein expression guided chemical profiling of living cells by the simultaneous observation of Raman scattering and anti-Stokes fluorescence emission. Scientific Reports. 7, 43569 (2017).
    DOI:10.1038/srep43569
  • 2016
  • 1.
    S. Lieb, T. Littmann, N. Plank, J. Felixberger, M. Tanaka, T. Schäfer, S. Krief, S. Elz, K. Friedland, G. Bernhardt, J. Wegener, T. Ozawa, A. Buschauer, Label-free versus conventional cellular assays: Functional investigations on the human histamine H1 receptor. Pharmacological Research. 114, 13–26 (2016).
    DOI:10.1016/j.phrs.2016.10.010
  • 1.
    T. Yamada, H. Yoshimura, R. Shimada, M. Hattori, M. Eguchi, T. K. Fujiwara, A. Kusumi, T. Ozawa, Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres. Scientific Reports. 6, 38910 (2016).
    DOI:10.1038/srep38910
  • 1.
    T. Frank, A. Reichel, O. Larsen, A.-C. Stilp, M. M. Rosenkilde, T. Stamminger, T. Ozawa, N. Tschammer, Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28. Cell Communication and Signaling. 14, 31 (2016).
    DOI:10.1186/s12964-016-0154-xPubMed:27955674
  • 1.
    O. Takenouchi, A. Kanno, H. Takakura, M. Hattori, T. Ozawa, Bioluminescent Indicator for Highly Sensitive Analysis of Estrogenic Activity in a Cell-Based Format. Bioconjugate Chemistry. 27, 2689–2694 (2016).
    DOI:10.1021/acs.bioconjchem.6b00466
  • 1.
    Y. Nasu, A. Benke, S. Arakawa, G. J. Yoshida, G. Kawamura, S. Manley, S. Shimizu, T. Ozawa, In Situ Characterization of Bak Clusters Responsible for Cell Death Using Single Molecule Localization Microscopy. Scientific Reports. 6, 27505 (2016).
    DOI:10.1038/srep27505
  • 1.
    M. Endo, M. Hattori, H. Toriyabe, H. Ohno, H. Kamiguchi, Y. Iino, T. Ozawa, Optogenetic activation of axon guidance receptors controls direction of neurite outgrowth. Scientific Reports. 6, 23976 (2016).
    DOI:10.1038/srep23976
  • 1.
    M. Hattori, G. Kawamura, R. Kojima, M. Kamiya, Y. Urano, T. Ozawa, Confocal Bioluminescence Imaging for Living Tissues with a Caged Substrate of Luciferin. Analytical Chemistry. 88, 6231–6238 (2016).
    DOI:10.1021/acs.analchem.5b04142
  • 1.
    Y. Nasu, Y. Asaoka, M. Namae, H. Nishina, H. Yoshimura, T. Ozawa, Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation. Analytical Chemistry. 88, 838–844 (2016).
    DOI:10.1021/acs.analchem.5b03367
  • 2015
  • 1.
    T. Littmann, M. Göttle, M. T. Reinartz, S. Kälble, I. W. Wainer, T. Ozawa, R. Seifert, Recruitment of β -Arrestin 1 and 2 to the β 2 -Adrenoceptor: Analysis of 65 Ligands. Journal of Pharmacology and Experimental Therapeutics. 355, 183–190 (2015).
    DOI:10.1124/jpet.115.227959
  • 1.
    T. Tamaru, M. Hattori, K. Honda, Y. Nakahata, P. Sassone-Corsi, G. T. J. van der Horst, T. Ozawa, K. Takamatsu, CRY Drives Cyclic CK2-Mediated BMAL1 Phosphorylation to Control the Mammalian Circadian Clock. PLOS Biology. 13, e1002293 (2015).
    DOI:10.1371/journal.pbio.1002293
  • 1.
    Y. Katsura, H. Kubota, K. Kunida, A. Kanno, S. Kuroda, T. Ozawa, An optogenetic system for interrogating the temporal dynamics of Akt. Scientific Reports. 5, 14589 (2015).
    DOI:10.1038/srep14589
  • 1.
    H. Segawa, Y. Kaji, P. Leproux, V. Couderc, T. Ozawa, T. Oshika, H. Kano, Multimodal and multiplex spectral imaging of rat cornea ex vivo using a white-light laser source. Journal of Biophotonics. 8, 705–713 (2015).
    DOI:10.1002/jbio.201400059PubMed:25378211
  • 1.
    M. Kodaka, Z. Yang, K. Nakagawa, J. Maruyama, X. Xu, A. Sarkar, A. Ichimura, Y. Nasu, T. Ozawa, H. Iwasa, M. Ishigami-Yuasa, S. Ito, H. Kagechika, Y. Hata, A new cell-based assay to evaluate myogenesis in mouse myoblast C2C12 cells. Experimental Cell Research. 336, 171–181 (2015).
    DOI:10.1016/j.yexcr.2015.06.015
  • 1.
    T. Nishiguchi, T. Yamada, Y. Nasu, M. Ito, H. Yoshimura, T. Ozawa, Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans. Journal of Biomedical Optics. 20, 101205 (2015).
    DOI:10.1117/1.JBO.20.10.101205
  • 1.
    H. SEGAWA, M. OKUNO, P. LEPROUX, V. COUDERC, T. OZAWA, H. KANO, Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source. Analytical Sciences. 31, 299–305 (2015).
    DOI:10.2116/analsci.31.299
  • 1.
    M. HATTORI, T. OZAWA, High-throughput Live Cell Imaging and Analysis for Temporal Reaction of G Protein-coupled Receptor Based on Split Luciferase Fragment Complementation. Analytical Sciences. 31, 327–330 (2015).
    DOI:10.2116/analsci.31.327
  • 1.
    A. Takamura, M. Hattori, H. Yoshimura, T. Ozawa, Simultaneous Time-Lamination Imaging of Protein Association Using a Split Fluorescent Timer Protein. Analytical Chemistry. 87, 3366–3372 (2015).
    DOI:10.1021/ac504583t
  • 1.
    M. T. Reinartz, S. Kälble, T. Littmann, T. Ozawa, S. Dove, V. Kaever, I. W. Wainer, R. Seifert, Structure-bias relationships for fenoterol stereoisomers in six molecular and cellular assays at the β2-adrenoceptor. Naunyn-Schmiedeberg’s Archives of Pharmacology. 388, 51–65 (2015).
    DOI:10.1007/s00210-014-1054-5
  • 2014
  • 1.
    S. Haga, T. Ozawa, Y. Yamada, N. Morita, I. Nagashima, H. Inoue, Y. Inaba, N. Noda, R. Abe, K. Umezawa, M. Ozaki, p62/SQSTM1 Plays a Protective Role in Oxidative Injury of Steatotic Liver in a Mouse Hepatectomy Model. Antioxidants &Redox Signaling. 21, 2515–2530 (2014).
    DOI:10.1089/ars.2013.5391
  • 1.
    S. A. Hoyng, S. Gnavi, F. de Winter, R. Eggers, T. Ozawa, A. Zaldumbide, R. C. Hoeben, J. A. M Malessy, J. Verhaagen, Developing a potentially immunologically inert tetracycline-regulatable viral vector for gene therapy in the peripheral nerve. Gene Therapy. 21, 549–557 (2014).
    DOI:10.1038/gt.2014.22PubMed:24694534
  • 1.
    H. Segawa, N. Fukutake, P. Leproux, V. Couderc, T. Ozawa, H. Kano, Electronically resonant third-order sum frequency generation spectroscopy using a nanosecond white-light supercontinuum. Optics Express. 22, 10416 (2014).
    DOI:10.1364/OE.22.010416
  • 1.
    M. Hirohama, A. R. D. Voet, T. Ozawa, H. Saitoh, Y. Nakao, K. Y. J. Zhang, A. Ito, M. Yoshida, Assay methods for small ubiquitin-like modifier (SUMO)–SUMO-interacting motif (SIM) interactions in vivo and in vitro using a split-luciferase complementation system. Analytical Biochemistry. 448, 92–94 (2014).
    DOI:10.1016/j.ab.2013.12.009
  • 1.
    K. Imamura, N. Imamachi, G. Akizuki, M. Kumakura, A. Kawaguchi, K. Nagata, A. Kato, Y. Kawaguchi, H. Sato, M. Yoneda, C. Kai, T. Yada, Y. Suzuki, T. Yamada, T. Ozawa, K. Kaneki, T. Inoue, M. Kobayashi, T. Kodama, Y. Wada, K. Sekimizu, N. Akimitsu, Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli. Molecular Cell (2014) (available at https://linkinghub.elsevier.com/retrieve/pii/S1097276514000410).
  • 2013
  • 1.
    M. Ito, T. Ozawa, S. Takada, Folding Coupled with Assembly in Split Green Fluorescent Proteins Studied by Structure-based Molecular Simulations. The Journal of Physical Chemistry B. 117, 13212–13218 (2013).
    DOI:10.1021/jp4032817
  • 1.
    G. Niu, L. Zhu, D. N. Ho, F. Zhang, H. Gao, Q. Quan, N. Hida, T. Ozawa, G. Liu, X. Chen, Longitudinal Bioluminescence Imaging of the Dynamics of Doxorubicin Induced Apoptosis. Theranostics. 3, 190–200 (2013).
    DOI:10.7150/thno.5825
  • 1.
    T. Tamaru, M. Hattori, Y. Ninomiya, G. Kawamura, G. Varès, K. Honda, D. P. Mishra, B. Wang, I. Benjamin, P. Sassone-Corsi, T. Ozawa, K. Takamatsu, ROS Stress Resets Circadian Clocks to Coordinate Pro-Survival Signals. PLoS ONE. 8, e82006 (2013).
    DOI:10.1371/journal.pone.0082006PubMed:24312621
  • 1.
    H. Inoue, T. Sakaue, T. Ozawa, S. Higashiyama, Spatiotemporal visualization of proHB-EGF ectodomain shedding in living cells. The Journal of Biochemistry. 154, 67–76 (2013).
    DOI:10.1093/jb/mvt030
  • 1.
    M. Hattori, M. Tanaka, H. Takakura, K. Aoki, K. Miura, T. Anzai, T. Ozawa, Analysis of temporal patterns of GPCR–β-arrestin interactions using split luciferase-fragment complementation. Molecular BioSystems. 9, 957 (2013).
    DOI:10.1039/c2mb25443c
  • 1.
    L. Yang, Y. Nasu, M. Hattori, H. Yoshimura, A. Kanno, T. Ozawa, Bioluminescent Probes to Analyze Ligand-Induced Phosphatidylinositol 3,4,5-Trisphosphate Production with Split Luciferase Complementation. Analytical Chemistry. 85, 11352–11359 (2013).
    DOI:10.1021/ac402278f
  • 1.
    M. Hattori, S. Haga, H. Takakura, M. Ozaki, T. Ozawa, Sustained accurate recording of intracellular acidification in living tissues with a photo-controllable bioluminescent protein. Proceedings of the National Academy of Sciences. 110, 9332–9337 (2013).
    DOI:10.1073/pnas.1304056110
  • 1.
    R. Kojima, H. Takakura, T. Ozawa, Y. Tada, T. Nagano, Y. Urano, Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo. Angewandte Chemie International Edition. 52, 1175–1179 (2013).
    DOI:10.1002/anie.201205151
  • 2012
  • 1.
    H. Takakura, R. Kojima, T. Ozawa, T. Nagano, Y. Urano, Development of 5′- and 7′-Substituted Luciferin Analogues as Acid-Tolerant Substrates of Firefly Luciferase. ChemBioChem. 13, 1424–1427 (2012).
    DOI:10.1002/cbic.201200142
  • 1.
    H. Yoshimura, A. Inaguma, T. Yamada, T. Ozawa, Fluorescent Probes for Imaging Endogenous β-Actin mRNA in Living Cells Using Fluorescent Protein-Tagged Pumilio. ACS Chemical Biology. 7, 999–1005 (2012).
    DOI:10.1021/cb200474a
  • 1.
    H. Takakura, M. Hattori, M. Takeuchi, T. Ozawa, Visualization and Quantitative Analysis of G Protein-Coupled Receptor−β-Arrestin Interaction in Single Cells and Specific Organs of Living Mice Using Split Luciferase Complementation. ACS Chemical Biology. 7, 901–910 (2012).
    DOI:10.1021/cb200360z
  • 1.
    T. Ishimoto, H. Mano, T. Ozawa, H. Mori, Measuring CREB Activation Using Bioluminescent Probes That Detect KID–KIX Interaction in Living Cells. Bioconjugate Chemistry. 23, 923–932 (2012).
    DOI:10.1021/bc200491j
  • 1.
    M. Ozaki, S. Haga, T. Ozawa, In Vivo Monitoring of Liver Damage Using Caspase-3 Probe. Theranostics. 2, 207–214 (2012).
    DOI:10.7150/thno.3806
  • 2011
  • 1.
    T. Tamaru, M. Hattori, K. Honda, I. Benjamin, T. Ozawa, K. Takamatsu, Synchronization of Circadian Per2 Rhythms and HSF1-BMAL1:CLOCK Interaction in Mouse Fibroblasts after Short-Term Heat Shock Pulse. PLoS ONE. 6, e24521 (2011).
    DOI:10.1371/journal.pone.0024521
  • 1.
    T. Yamada, H. Yoshimura, A. Inaguma, T. Ozawa, Visualization of Nonengineered Single mRNAs in Living Cells Using Genetically Encoded Fluorescent Probes. Analytical Chemistry. 83, 5708–5714 (2011).
    DOI:10.1021/ac2009405
  • 1.
    T. Ishimoto, T. Ozawa, H. Mori, Real-Time Monitoring of Actin Polymerization in Living Cells Using Split Luciferase. Bioconjugate Chemistry. 22, 1136–1144 (2011).
    DOI:10.1021/bc100595z
  • 1.
    A. K. M. Kafi, M. Hattori, N. Misawa, T. Ozawa, Dual-Color Bioluminescence Analysis for Quantitatively Monitoring G-Protein-Coupled Receptor and β-Arrestin Interactions. Pharmaceuticals. 4, 457–469 (2011).
    DOI:10.3390/ph4030457
  • 2010
  • 1.
    S. Haga, N. Morita, K. Irani, M. Fujiyoshi, T. Ogino, T. Ozawa, M. Ozaki, p66Shc has a pivotal function in impaired liver regeneration in aged mice by a redox-dependent mechanism. Laboratory Investigation. 90, 1718–1726 (2010).
    DOI:10.1038/labinvest.2010.119
  • 1.
    M. Takeuchi, Y. Nagaoka, T. Yamada, H. Takakura, T. Ozawa, Ratiometric Bioluminescence Indicators for Monitoring Cyclic Adenosine 3′,5′-Monophosphate in Live Cells Based on Luciferase-Fragment Complementation. Analytical Chemistry. 82, 9306–9313 (2010).
    DOI:10.1021/ac102692u
  • 1.
    S. B. Kim, T. Ozawa, Creating bioluminescent indicators to visualise biological events in living cells and animals. Supramolecular Chemistry. 22, 440–449 (2010).
    DOI:10.1080/10610278.2010.485251
  • 1.
    N. Misawa, A. K. M. Kafi, M. Hattori, K. Miura, K. Masuda, T. Ozawa, Rapid and High-Sensitivity Cell-Based Assays of Protein−Protein Interactions Using Split Click Beetle Luciferase Complementation: An Approach to the Study of G-Protein-Coupled Receptors. Analytical Chemistry. 82, 2552–2560 (2010).
    DOI:10.1021/ac100104q
  • 1.
    A. K. M. KAFI, M. HATTORI, T. OZAWA, LUCIFERASES FOR THE STUDY OF PROTEIN–PROTEIN INTERACTIONS IN LIVE CELLS AND ANIMALS. Nano LIFE. 1, 79–87 (2010).
    DOI:10.1142/S1793984410000079
  • 2009
  • 1.
    N. Hida, M. Awais, M. Takeuchi, N. Ueno, M. Tashiro, C. Takagi, T. Singh, M. Hayashi, Y. Ohmiya, T. Ozawa, High-Sensitivity Real-Time Imaging of Dual Protein-Protein Interactions in Living Subjects Using Multicolor Luciferases. PLoS ONE. 4, e5868 (2009).
    DOI:10.1371/journal.pone.0005868
  • 1.
    T. Ozawa, Imaging biological molecules using novel optical probes. Photomedicine and photobiology. 31, 27–28 (2009).
  • 2007
  • 1.
    A. Kanno, Y. Yamanaka, H. Hirano, Y. Umezawa, T. Ozawa, Cyclic Luciferase for Real-Time Sensing of Caspase-3 Activities in Living Mammals. Angewandte Chemie International Edition. 46, 7595–7599 (2007).
    DOI:10.1002/anie.200700538
  • 1.
    S. B. Kim, A. Kanno, T. Ozawa, H. Tao, Y. Umezawa, Nongenomic Activity of Ligands in the Association of Androgen Receptor with Src. ACS Chemical Biology. 2, 484–492 (2007).
    DOI:10.1021/cb7000439
  • 1.
    T. Ozawa, Y. Natori, M. Sato, Y. Umezawa, Imaging dynamics of endogenous mitochondrial RNA in single living cells. Nature Methods. 4, 413–419 (2007).
    DOI:10.1038/nmeth1030
  • 1.
    S. B. Kim, T. Ozawa, H. Tao, Y. Umezawa, A proinflammatory cytokine sensor cell for assaying inflammatory activities of nanoparticles. Analytical Biochemistry. 362, 148–150 (2007).
    DOI:10.1016/j.ab.2006.11.004
  • 1.
    T. Ozawa, Y. Natori, Y. Sako, H. Kuroiwa, T. Kuroiwa, Y. Umezawa, A Minimal Peptide Sequence That Targets Fluorescent and Functional Proteins into the Mitochondrial Intermembrane Space. ACS Chemical Biology. 2, 176–186 (2007).
    DOI:10.1021/cb600492a
  • 1.
    M. TAKEUCHI, T. OZAWA, Methods for Imaging and Analyses of Intracellular Organelles Using Fluorescent and Luminescent Proteins. Analytical Sciences. 23, 25–29 (2007).
    DOI:10.2116/analsci.23.25
  • 2006
  • 1.
    S. B. Kim, Y. Natori, T. Ozawa, Y. Umezawa, H. Tao, A method for determining the activities of cytokines based on the nuclear transport of nuclear factor-κB. Analytical Biochemistry. 359, 147–149 (2006).
    DOI:10.1016/j.ab.2006.07.013
  • 1.
    A. Kanno, T. Ozawa, Y. Umezawa, Genetically Encoded Optical Probe for Detecting Release of Proteins from Mitochondria toward Cytosol in Living Cells and Mammals. Analytical Chemistry. 78, 8076–8081 (2006).
    DOI:10.1021/ac061488a
  • 1.
    A. Kanno, T. Ozawa, Y. Umezawa, Intein-Mediated Reporter Gene Assay for Detecting Protein−Protein Interactions in Living Mammalian Cells. Analytical Chemistry. 78, 556–560 (2006).
    DOI:10.1021/ac051451a
  • 1.
    T. Ozawa, Designing split reporter proteins for analytical tools. Analytica Chimica Acta. 556, 58–68 (2006).
    DOI:10.1016/j.aca.2005.06.026
  • 2005
  • 1.
    S. B. Kim, T. Ozawa, Y. Umezawa, A genetically encoded indicator for assaying bioactive chemicals that induce nuclear transport of glucocorticoid receptor. Analytical Biochemistry. 347, 213–220 (2005).
    DOI:10.1016/j.ab.2005.09.011
  • 1.
    S. B. Kim, R. Takao, T. Ozawa, Y. Umezawa, Quantitative Determination of Protein Nuclear Transport Induced by Phosphorylation or by Proteolysis. Analytical Chemistry. 77, 6928–6934 (2005).
    DOI:10.1021/ac050966z
  • 1.
    S. B. Kim, T. Ozawa, Y. Umezawa, Genetically Encoded Stress Indicator for Noninvasively Imaging Endogenous Corticosterone in Living Mice. Analytical Chemistry. 77, 6588–6593 (2005).
    DOI:10.1021/ac0510078
  • 1.
    T. Ozawa, Methods of Analysis for Protein Dynamics in Living Cells Based on Protein Splicing. Bulletin of the Chemical Society of Japan. 78, 739–751 (2005).
    DOI:10.1246/bcsj.78.739
  • 1.
    T. Ozawa, A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells. Nucleic Acids Research. 33, e34–e34 (2005).
    DOI:10.1093/nar/gni032
  • 2004
  • 1.
    S. B. Kim, T. Ozawa, S. Watanabe, Y. Umezawa, High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. Proceedings of the National Academy of Sciences. 101, 11542–11547 (2004).
    DOI:10.1073/pnas.0401722101
  • 2003
  • 1.
    A. Kaihara, Y. Kawai, M. Sato, T. Ozawa, Y. Umezawa, Locating a Protein−Protein Interaction in Living Cells via Split Renilla Luciferase Complementation. Analytical Chemistry. 75, 4176–4181 (2003).
    DOI:10.1021/ac0300800
  • 1.
    S. B. KIM, T. OZAWA, Y. UMEZAWA, A Screening Method for Estrogens Using an Array-Type DNA Glass Slide. Analytical Sciences. 19, 499–504 (2003).
    DOI:10.2116/analsci.19.499
  • 1.
    T. Ozawa, Y. Sako, M. Sato, T. Kitamura, Y. Umezawa, A genetic approach to identifying mitochondrial proteins. Nature Biotechnology. 21, 287–293 (2003).
    DOI:10.1038/nbt791
  • 2002
  • 1.
    Y. Umezawa, T. Ozawa, M. Sato, Probing Chemical Processes in Living Cells: Applications for Assay and Screening of Chemicals that Disrupt Cellular Signaling Pathways. Bulletin of the Chemical Society of Japan. 75, 1423–1433 (2002).
    DOI:10.1246/bcsj.75.1423
  • 1.
    T. Ozawa, Y. Umezawa, Peptide Assemblies in Living Cells. Methods for Detecting Protein-Protein Interactions†. Supramolecular Chemistry. 14, 271–280 (2002).
    DOI:10.1080/10610270290026185
  • 1.
    M. Sato, T. Ozawa, K. Inukai, T. Asano, Y. Umezawa, Fluorescent indicators for imaging protein phosphorylation in single living cells. Nature Biotechnology. 20, 287–294 (2002).
    DOI:10.1038/nbt0302-287
  • 2001
  • 1.
    T. Ozawa, M. Takeuchi, A. Kaihara, M. Sato, Y. Umezawa, Protein Splicing-Based Reconstitution of Split Green Fluorescent Protein for Monitoring Protein−Protein Interactions in Bacteria: Improved Sensitivity and Reduced Screening Time. Analytical Chemistry. 73, 5866–5874 (2001).
    DOI:10.1021/ac010717k
  • 1.
    K. Sasaki, T. Ozawa, Y. Umezawa, Screening method for antagonists that inhibit the binding of calmodulin to a target peptide using surface plasmon resonance. Analytica Chimica Acta. 447, 63–74 (2001).
    DOI:10.1016/S0003-2670(01)01290-9
  • 1.
    T. Ozawa, Y. Umezawa, Detection of protein–protein interactions in vivo based on protein splicing. Current Opinion in Chemical Biology. 5, 578–583 (2001).
    DOI:10.1016/S1367-5931(00)00244-1
  • 1.
    J. Nakanishi, T. Nakajima, M. Sato, T. Ozawa, K. Tohda, Y. Umezawa, Imaging of Conformational Changes of Proteins with a New Environment-Sensitive Fluorescent Probe Designed for Site-Specific Labeling of Recombinant Proteins in Live Cells. Analytical Chemistry. 73, 2920–2928 (2001).
    DOI:10.1021/ac001528pPubMed:11467536
  • 1.
    T. Ozawa, A. Kaihara, M. Sato, K. Tachihara, Y. Umezawa, Split Luciferase as an Optical Probe for Detecting Protein−Protein Interactions in Mammalian Cells Based on Protein Splicing. Analytical Chemistry. 73, 2516–2521 (2001).
    DOI:10.1021/ac0013296
  • 2000 and before
  • 1.
    M. Sato, N. Hida, T. Ozawa, Y. Umezawa, Fluorescent Indicators for Cyclic GMP Based on Cyclic GMP-Dependent Protein Kinase Iα and Green Fluorescent Proteins. Analytical Chemistry. 72, 5918–5924 (2000).
    DOI:10.1021/ac0006167
  • 1.
    T. Ozawa, M. Fukuda, M. Nara, A. Nakamura, Y. Komine, K. Kohama, Y. Umezawa, How Can Ca 2+ Selectively Activate Recoverin in the Presence of Mg 2+ ? Surface Plasmon Resonance and FT-IR Spectroscopic Studies †. Biochemistry. 39, 14495–14503 (2000).
    DOI:10.1021/bi001930y
  • 1.
    Z. Quan, T. Ozawa, M. Sato, Y. Umezawa, Screening method for substrates of multidrug resistance-associated protein. Analytica Chimica Acta. 423, 197–203 (2000).
    DOI:10.1016/S0003-2670(00)01116-8
  • 1.
    T. Ozawa, S. Nogami, M. Sato, Y. Ohya, Y. Umezawa, A Fluorescent Indicator for Detecting Protein−Protein Interactions in Vivo Based on Protein Splicing. Analytical Chemistry. 72, 5151–5157 (2000).
    DOI:10.1021/ac000617z
  • 1.
    M. Mori, T. Konno, T. Ozawa, M. Murata, K. Imoto, K. Nagayama, Novel Interaction of the Voltage-Dependent Sodium Channel (VDSC) with Calmodulin: Does VDSC Acquire Calmodulin-Mediated Ca 2+ -Sensitivity? †. Biochemistry. 39, 1316–1323 (2000).
    DOI:10.1021/bi9912600
  • 1.
    T. Yoshida, M. Sato, T. Ozawa, Y. Umezawa, An SPR-Based Screening Method for Agonist Selectivity for Insulin Signaling Pathways Based on the Binding of Phosphotyrosine to Its Specific Binding Protein. Analytical Chemistry. 72, 6–11 (2000).
    DOI:10.1021/ac990795w
  • 1.
    T. Ozawa, K. Sasaki, Y. Umezawa, Metal ion selectivity for formation of the calmodulin–metal–target peptide ternary complex studied by surface plasmon resonance spectroscopy. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1434, 211–220 (1999).
    DOI:10.1016/S0167-4838(99)00185-5
  • 1.
    M. Sato, T. Ozawa, T. Yoshida, Y. Umezawa, A Fluorescent Indicator for Tyrosine Phosphorylation-Based Insulin Signaling Pathways. Analytical Chemistry. 71, 3948–3954 (1999).
    DOI:10.1021/ac990318d
  • 1.
    T. Ozawa, M. Sato, M. Sugawara, Y. Umezawa, An Assay Method for Evaluating Chemical Selectivity of Agonists for Insulin Signaling Pathways Based on Agonist-Induced Phosphorylation of a Target Peptide. Analytical Chemistry. 70, 2345–2352 (1998).
    DOI:10.1021/ac971192s
  • 1.
    T. Ozawa, M. Kakuta, M. Sugawara, Y. Umezawa, M. Ikura, An Optical Method for Evaluating Ion Selectivity for Calcium Signaling Pathways in the Cell. Analytical Chemistry. 69, 3081–3085 (1997).
    DOI:10.1021/ac9613141