Dr. Takeaki OZAWA

Research and Professional Experience

1998–2002
 		 Research Associate at Department of Chemistry, School of Science, The University of Tokyo
2002–2005
 		 Lecturer at Department of Chemistry, School of Science, The University of Tokyo
2005–2007
 		 Associate Professor, Department of Molecular Structure, Institute for Molecular Science
2007–
 		 Professor, Department of Chemistry, School of Science, The University of Tokyo
2014–
 		 Professor, Department of Biological Sciences, Graduate School of Science, The University of Tokyo


Awards

2011 JSPS Prize (Japan Society for the Promotion of Science)
2005 Young Scientists Award from Minister of MEXT
(Ministry of Education, Culture, Science and Technology)
2004  The Chemical Society of Japan Award for Young Chemists


Research Interests
   Analytical Chemistry, Protein Chemistry

Extramural Activities
　Scientific Reports (Springer Nature), Editorial Board Member
   Editor of Analytical Sciences (Journal of Japan Society for Analytical Chemistry)
   Editorial Advisory Board Member of Analytical Chemistry (ACS Journal)
   Advisory Board Member of Analyst (RSC Journal)

Research Subjects
   (1) Development of methods for imaging biomolecules in living subjects
   (2) Development of methods for controlling biomolecules in living cells

Selected Publications

  • Protein expression guided chemical profiling of living cells by the simultaneous observation of Raman scattering and anti-Stokes 
    fluorescence emission.
    L.-D. Chiu, T. Ichimura, T. Sekiya, H. Machiyama, T. Watanabe, H. Fujita, T. Ozawa, and K. Fujita,
    Sci. Rep., in press.

  • Spatiotemporal analysis with a genetically encoded fluorescent RNA probe 
    reveals TERRA function around telomeres.
    T. Yamada, H. Yoshimura, R. Shimada, M. Hattori, M. Eguchi, T. K. Fujiwara, A. Kusumi, T. Ozawa,
    Sci. Rep. 2016, 6, 38910.

  • Optogenetic activation of axon guidance receptors controls direction of 
    neurite outgrowth.
    M. Endo, M. Hattori, H. Toriyabe, H. Ohno, H. Kamiguchi, Y. Iino, T. Ozawa,
    Sci. Rep., 2016, 6, 23976.

  • In Situ Characterization of Bak Clusters Responsible for Cell Death 
    Using Single Molecule Localization Microscopy.
    Y. Nasu, A. Benke, S. Arakawa, G. J. Yoshida, G. Kawamura, S. Manley, S. 
    Shimizu, and T Ozawa,
    Sci. Rep.,2016, 6, 27505.

  • An optogenetic system for interrogating the temporal dynamics of Akt.
    Y. Katsura, H. Kubota, K. Kunida, A. Kanno, S. Kuroda, T. Ozawa,
    Sci. Rep.    , 2015, 5, 14589.

  • Sustained Accurate Recording of Intracellular Acidification in Living
    Tissues with a Photo-controllable Bioluminescent Protein.
    M. Hattori, S. Haga, H. Takakura, M. Ozaki and T. Ozawa,
    Proc. Nat. Acad. Sci. USA, 2013, 110, 9332-9337.

  • Cyclic Luciferase for Real-Time Sensing of Caspase-3 Activities in Living Mammals,
    A. Kanno, Y. Yamanaka, H. Hirano, Y. Umezawa and T. Ozawa,
    Angew. Chem. Int. Ed. 2007, 46, 7595.

  • Imaging Dynamics of Endogenous Mitochondrial RNA in Single Living Cells, T. Ozawa, Y. Natori, M. Sato and Y. Umezawa,
    Nature Methods, 2007, 4, 413.

  • High-Throughput Sensing and Noninvasive Imaging of Protein Nuclear Transport by Using Reconstitution of Split Renilla Luciferase,
    S. B. Kim, T. Ozawa, S. Watanabe, Y. Umezawa,
    Proc. Natl. Acad. Sci. USA., 2004, 101, 11542.

  • A genetic Approach to Identifying Mitochondrial Proteins,
    T. Ozawa, Y. Sako, M. Sato, T. Kitamura, and Y. Umezawa,
    Nature Biotechnol., 2003, 21, 287.

  • Fluorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells,
    M. Sato, T. Ozawa, K. Inukai, T. Asano, and Y. Umezawa,
    Nature Biotechnol., 2002, 20, 287.

  • Split Luciferase as an Optical Probe for Detecting Protein–Protein Interactions in Mammalian Cells Based on Protein Splicing,
    T. Ozawa, A. Kaihara, M. Sato, K. Tachihara and Y. Umezawa,
    Anal. Chem., 2001, 73, 2516.