ISPAC2016 Lecture Award (International Symposium on Pure and Applied Chemistry, Kuching, Malaysia)
2015
RSC Best Presentation Award (Royal Society of Chemistry Tokyo International Conference 2015)
2012
日本化学会 第92回春季年会 優秀講演賞(学術)
2006
日本化学会 第86回春季年会 学生講演賞
専門
生体分析化学・生物物理学・生体分子科学
主な研究テーマ
生細胞内分子の動態解析と操作を通じた生命現象作動機構の解明
主なターゲット:シグナル伝達、mRNA、機能性RNA
研究キーワード
1分子イメージング
光学顕微鏡
ライブイメージング
蛍光プローブ
生物発光
分子設計
分子操作
RNA
シグナル伝達
生体分子集合体
Peer-Reviewd Papers
G. Ado, N. Noda, H. T. Vu, A. D. Mahapatra, K. P. Arista, H. Yoshimura, D. M. Packwood, F. Ishidate, S. Sato, T. Ozawa and M. Uesugi,
Discovery of a phase-separating small molecule that selectively sequesters tubulin in cells. Chem. Sci., 13, 5760-5766 (2022). DOI: doi: 10.1039/d1sc07151c
Triple-color single-molecule imaging for analysis of the role of receptor oligomers in signal transduction.
H. Yoshimura*
* corresponding author Biophys. Physicobiol., 19, 1-9 (2022). DOI: 10.2142/biophysico.bppb-v19.0007
A Series of Furimazine Derivatives for Sustained Live-cell Bioluminescence Imaging and Application to the Monitoring of Myogenesis at Single-cell Level.
Mariko Orioka, Masatoshi Eguchi, Yuki Mizui, Yuma Ikeda Akihiro Sakama, Qiaojing Li, Hideaki Yoshimura*, Takeaki Ozawa, Daniel Citterio, Yuki Hiruta*
*co-corresponding author Bioconjugate Chem., 33, 496-504 (2022). DOI: 10.1021/acs.bioconjchem.2c00035
Long-term single cell bioluminescence imaging with C-3 position protected coelenterazine analogues.
Y. Mizui, M. Eguchi, M. Tanaka, Y. Ikeda, H. Yoshimura*, T. Ozawa, D. Citterio, Y. Hiruta*
* corresponding author Org. Biomol. Chem., 19, 579-586 (2021). DOI: 10.1039/d0ob02020f
Potential of Single-Molecule Live-Cell Imaging for Chemical Translational Biology.
H Yoshimura*
* corresponding author ChemBioChem, 22, 2941-2945 (2021). DOI: 10.1002/cbic.202100258
Signaling activations through G-protein-coupled-receptor aggragations.
M. Watabe, H. Yoshimura, S. N. V. Arjunan, K. Kaizu, K. Takahashi Phys. Rev. E, 102, 032413 (2020). DOI:10.1103/PhysRevE.102.032413
Synergetic roles of Formyl Peptide Receptor 1 oligomerization in ligand-induced signal transduction.
T. Nishiguchi, H. Yoshimura, R.S. Kasai, T. K. Fujiwara, T. Ozawa ACS Chem. Biol., 15, 2577-2587 (2020). DOI: 10.1021/acschembio.0c00631
Photocleavable Cadherin Inhibits Cell-to-Cell Mechanotransduction by Light.
M. Endo, T. Iwawaki, H. Yoshimura, and T. Ozawa ACS Chem. Biol., 14, 2206-2214 (2019). DOI: 10.1021/acschembio.9b00460
A Robust Split-Luciferase-Based Cell Fusion Screening for
Discovering Myogenesis-Promoting Molecules
Q. Li, H. Yoshimura, M. Komiya, K. Tajiri, M. Uesugi, Y. Hata, T. Ozawa Analyst, 143, 3472-3480 (2018). DOI: 10.1039/C8AN00285A
Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers.
O. Takenouchi, H. Yoshimura, T. Ozawa Sci. Rep., 8, 677 (2018). DOI: 10.1038/s41598-017-19130-y
Protein dynamics of the oxygen sensor protein HemAT as revealed by time-resolved step-scan FTIR spectroscopy.
A. Pavlou, H. Yoshimura, S. Aono, E. Pinakoulaki Biophys. J., 114, 584-591 (2018). DOI: 10.1016/j.bpj.2017.12.012
Live Cell Imaging of Endogenous RNAs Using Pumilio Homology Domain Mutants: Principles and Applications.
H. Yoshimura*
*corresponding author Biochemistry, 57, 200-208 (2018). DOI: 10.1021/acs.biochem.7b00983
Real-time fluorescence imaging of single-molecule endogenous non-coding
RNA in living cells.
H. Yoshimura and T. Ozawa, Methods Mol. Biol., 1649, 337-347 (2018).DOI: 10.1007/978-1-4939-7213-5_22
Probing the role of the heme distal and proximal environment in ligand dynamics in the signal transducer protein HemAT by time-resolved step-scan FTIR and resonance Raman spectroscopy.
A. Pavlou, A. Loullis, H. Yoshimura, S. Aono, E. Pinakoulaki, Biochemistry, 56, 5309-5317 (2017). DOI: 10.1021/acs.biochem.7b00558
Spatiotemporal analysis with a genetically encoded fluorescent RNA probe
reveals TERRA function around telomeres.
T. Yamada, H. Yoshimura, R.
Shimada, M. Hattori, M. Eguchi, T. K. Fujiwara, A. Kusumi, T. Ozawa, Sci. Rep.6, 38910 (2016). DOI: 10.1038/srep38910
Monitoring of RNA dynamics in living cells using PUM-HD and fluorescent protein reconstitution technique.
H. Yoshimura and T. Ozawa, Methods Enzymol., 572, 65-85 (2016). DOI: 10.1016/bs.mie.2016.03.018
Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation.
Y. Nasu, Y. Asaoka, M. Namae, H. Nishina, H. Yoshimura, T. Ozawa,
Anal. Chem., 88, 838-844 (2016). DOI: 10.1021/acs.analchem.5b03367
Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans
T. Nishiguchi, T. Yamada, Y. Nasu, M. Ito, H. Yoshimura, T. Ozawa J. Biomed. Opt.,20, 101205 (2015).DOI: 10.1117/1.jbo.20.10.101205
Simultaneous time-lamination imaging of protein association using a split fluorescent timer protein.
A. Takamura, M Hattori, H. Yoshimura, T Ozawa Anal. Chem.,87, 3366-3372 (2015). DOI: 10.1021/ac504583t
Method of split-reporter reconstitution for the analysis of biomolecules
H. Yoshimura, T. Ozawa Chem. Rec, 14, 492-501 (2014). DOI: 10.1002/tcr.201402001
Bioluminescent Probes to Analyze Ligand-induced Phosphatidylinositol
3,4,5-trisphosphate Production with Split Luciferase Complementation
L.Z. Yang, Y. Nasu, M. Hattori, H. Yoshimura, A. Kanno, T. Ozawa, Anal. Chem., 85, 11352-11359 (2013). DOI: 10.1021/ac402278f
Advances in fluorescence and bioluminescence imaging
T. Ozawa, H.
Yoshimura and S.B. Kim, Anal. Chem.,85, 590-609 (2013). DOI: 10.1021/ac3031724
Fluorescent probes for imaging endogenous β-actin mRNA in living
cells using fluorescent protein-tagged pumilio.
H. Yoshimura, A. Inaguma, T. Yamada and T. Ozawa ACS Chem. Biol., 7, 999-1005 (2012). DOI: 10.1021/cb200474a
Visualization of non-engineered single mRNAs in living cells using genetically encoded fluorescent probes.
T. Yamada, H. Yoshimura, A. Inaguma and T. Ozawa, Anal. Chem., 83, 5708-5714 (2011). DOI: 10.1021/ac2009405
Hydrogen bonding interaction on the heme-bound ligand in the heme-based O2 sensor protein, M. Nishimura, H. Yoshimura, K. Ozawa, S. Yoshioka, M. Kubo, T. Kitagawa and S. Aono, J. PorphyrinsPhthalocyanines, 12, 142-148 (2008). DOI: 10.1142/S1088424608000182
Protein conformation changes of HemAT-Bs upon ligand binding probed by ultraviolet resonance Raman spectroscopy. S. F. EI-Mashtoly, Y. Gu, H. Yoshimura, S. Yoshioka, S. Aono, T. Kitagawa, J. Biol. Chem., 283, 6942-6946 (2008). DOI: 10.1074/jbc.M709209200
The signal transduction mechanism of HemAT-Bs through the proximal heme pocket revealed by time-resolved resonance Raman spectroscopy. H. Yoshimura, S. Yoshioka, Y. Mizutani and S. Aono, Biochem. Biophys. Res. Commun., 307, 1053-1057 (2007). DOI: 10.1016/j.bbrc.2007.04.041
Two ligand binding sites in the O2-sensing signal transducer HemAT: implications for ligand recognition/discrimination and signaling, E. Pinakoulaki, H. Yoshimura, V. Daskalakis, S. Yoshioka, S. Aono and C. Varotsis, Proc. Natl. Acad. Sci. USA, 103, 14796-14801 (2006). DOI: 10.1073/pnas.0604248103
Specific hydrogen-bonding networks responsible for selective O2 sensing of the oxygen sensor protein HemAT from Bacillussubtilis, H. Yoshimura, S. Yoshioka, K. Kobayashi, T. Ohta, T. Uchida, M. Kubo, T. Kitagawa and S. Aono, Biochemistry, 45, 8301-8307 (2006). DOI: 10.1021/bi060315c
Recognition and discrimination of gases by the oxygen-sensing signal transducer protein HemAT as revealed by FTIR spectroscopy, E. Pinakoulaki, H. Yoshimura, S. Yoshioka, S. Aono and C. Varotsis, Biochemistry, 45, 7763-7766 (2006). DOI: 10.1021/bi0604072
Non-covalent modification of the heme-pocket of apomyoglobin by a 1,10-phenanthroline derivative, Y. Hitomi, H. Mukai, H. Yoshimura, T. Tanaka and T. Funabiki, Bioorg. Med. Chem. Lett., 16, 248-251 (2006). DOI: 10.1016/j.bmcl.2005.10.016
Biophysical properties of a c-type heme in chemotaxis signal transducer protein DcrA, S. Yoshioka, K. Kobayashi, H. Yoshimura, T. Uchida, T. Kitagawa and S. Aono, Biochemistry, 44, 15406.-15413 (2005). DOI: 10.1021/bi0513352
Oxygen-sensing mechanism of HemAT from Bacillussubtilis: a resonance Raman spectroscopic study,
T. Ohta, H. Yoshimura, S. Yoshioka, S. Aono and Teizo Kitagawa, J. Am. Chem. Soc., 126, 15000-15001 (2004). DOI: 10.1021/ja046896f
著書・総説・解説
Optical monitoring of single molecule dynamics of RNA in living cells
Hideaki Yoshimura*, Takeaki Ozawa
* corresponding author
Springer Series in Chemical Physics,"Progress in Photon Science", 95-106 (2021)
A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules
Q. Li, H Yoshimura*, T. Ozawa
* corresponding author Methods in Mol. Biol., 2274, 79-87 (2021). DOI: 10.1007/978-1-0716-1258-3_8
Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools
O. Takenouchi, H Yoshimura*, T. Ozawa
* corresponding author Methods in Mol. Biol., 2274, 15-23 (2021). DOI: 10.1007/978-1-0716-1258-3_2
Optical Control of G Protein-Coupled Receptor Activated in Living Cells
Hideaki Yoshimura, Takeaki Ozawa
Springer Series in Chemical Physics,"Progress in Photon Science: Recent Advances", Chapter 7, pp.129-138, Springer (2018). DOI: 10.1007/978-3-030-05974-3_7
生命分子の機能を超えるための解析化学
吉村英哲、小澤岳昌
CSJ Current Review 30 生命機能に迫る分子科学:生命分子を真似る、飾る、超える(化学同人社刊)第2章 p28-33, 2018
"Oligomer Formation and Signal Transduction of GPCR -A Single-Molecule Live Imaging Study"
14th International Symposium on Nanomedicine, Taiwan-Japan-Korea Nanomedicine Meeting (2021年11月19日 online)
"The roles of receptor oligomerization for signal transduction - A study through single-molecule live-cell imaging-"
13th International Symposium on Nanomedicine (2019年12月5日 甲南大学ポートアイランドキャンパス)
"A single molecule imaging approach to understand signal transduction on the plasma membrane in living cells"
12th International Symposium on Nanomedicine (2018年12月7日 山口大学小串キャンパス)
"Simultaneous single molecule observation of telomeric-repeat containing RNA and proteins in living cells"
CMCB2017 (2017年4月26日 Hilton Xi'an, China)
"Simultaneous single molecule imaging of non-coding RNA and proteins in living cells"
AnalytiX 2017 (2017年3月24日 福岡)
"Single molecule imaging in living cells to reveal the relationship between motions and functions of biological molecules"
10th International Symposium on Nanomedicine(2016年11月24日 産業技術総合研究所)
"Single molecule imaging approach to reveal molecular motions and functions in cellular events"
1st Nano/Bioscience International Syposium
(2016年10月7日 同志社大学)
"Tailor-made design of protein-based probes to visualize and analyze single-molecule motion of RNAs in living cells
"
International Symposium on Pure and Applied Chemistry 2016 (ISPAC2016)
(2016年8月16日 Borneo Convention Center Kuching, Malaysia)
"Analysis of distribution and motion of a non-coding RNA in living cells using a single molecule tracking approach"
The 5th Serendipiter seminar (JST-ImPACT program)
(2016年7月26日 東京大学)
"Single molecule imaging of telomeric-repeat containing RNA in living cells"
9th International Symposium on Nanomedicine(2015年12月12日 三重大学)
"Single molecule imaging to reveal mechanisms of complex cellular systems"
NTU-SNU-UT Chemistry Symposium(2015年1月16日 National Taiwan University)
"Single molecule imaging in living cells to reveal the relationship between motions and functions of biological molecules"
8th International Symposium on Nanomedicine(2014年12月6日 愛媛大学)
"A study of a molecular mechanism of intracellular signal transduction based on single-molecule imaging"
The 2nd Japan-China Symposium on Nanomedicine(2014年5月17日 広島大学霞キャンパス)
"Analysis of RNA dynamics in living cells based on single molecule imaging"
7th International Symposium on Nanomedicine(2013年11月9日 九州工業大学)
"Visualization of molecular motion in living cells using a single molecule imaging method"
錯体化学会第63回討論会(2013年11月3日 琉球大学千原キャンパス)